On-Demand Mass Spectrometry Analysis by Miniature Mass Spectrometer

Electrospray ionization (ESI) has become a powerful tool for the analysis of biomolecules by mass spectrometry (MS). The process ESI is difficult to control, and side reactions such as electrochemical can occur during because high voltages applied. Herein, novel on-demand MS method was developed based on discontinuous ion injection-induced miniature system. Highly efficient enabled under low (<300 V) using atmospheric pressure interface. On-demand showed comparable sensitivity with regular nanoESI analyses series compounds. It found be softer than or methods proteins myoglobin cytochrome C. As finished soon interface closed, sample consumption observed reduce significantly analysis, allowing single-cell multiple MS/MS measurements.